Publications from Chen's Group

2008

50. Cheng, J.; Yu, H.; Lau, K.; Huang, S.; Chokhawala, H. A.; Li, Y.; Tiwari, V. K.; *Chen, X. Multifunctionality of Campylobacter jejuni sialyltransferase CstII: Characterization of GD3/GT3 oligosaccharide synthase, GD3 oligosaccharide sialidase and trans-sialidase activities. Glycobiology 2008, In press.

49. Wang, Z.; Gilbert, M.; Eguchi, H.; Yu, H.; Cheng, J.; Muthana, S.; Zhou, L.; Wang, P. G.; Chen, X.; *Huang, X. Chemoenzymatic synthesis of tumor-associated carbohydrate antigen Globo-H and stage-specific embryonic antigen 4. Adv. Synth. Catal. 2008, In press.

48. Yu, H.; *Chen X. Enzymatic synthesis of carbohydrate-containing biomolecules. In Wiley Encyclopedia of Chemical Biology. Chief Advisor: Tadhag P. Begley, John Wiley & Sons, Inc. NJ, 2008. In press.

47. Yu, H.; Chokhawala, H. A.; Huang, S.; *Chen, X. Chemoenzymatic synthesis of sialosides and their applications. In Chemical Glycobiology ACS book series, Chen, X.; Halcomb, R.; Wang, P. G. Eds 990, Chen, X.; Halcomb, R.; Wang, P. G. Eds. 2008, Oxford University Press, Page 79-98, In press.

46. Li, Y.; Yu, H.; Cao, H.; Lau, K.; Muthana, S.; Tiwari, V. K.; Son, B., *Chen, X. Pasteurella multocida sialic acid aldolase: a promising biocatalyst. Appl. Microbiol. Biotech. 2008, DOI 10.1007/s00253-008-1506-2, In press.

45. Linman, M. J.; Taylor, J. D.; Yu, H.; Chen, X.; *Cheng, Q. Surface Plasmon Resonance study of protein-carbohydrate interactions using biotinylated sialosides. Anal. Chem. 2008, DOI 10.1021/ac7002566e, In press.

44. Sun, M.; Li, Y.; Chokhawala, H. A.; Henning, R.; *Chen, X. N-terminal 112 amino acid residues are not required for the sialyltransferase activity of /Photobacterium damsela/ a2,6-sialyltransferase. Biotechnol. Lett. 2008, 30, 671-676.[Full Text]

2007

43. Muthana, S.; Yu, H.; Huang, S.; *Chen, X. Chemoenzymatic synthesis of size-defined polysaccharides by sialyltransferase-catalyzed block transfer of oligosaccharides. J. Am. Chem. Soc. 2007, 129, 11918-11919. [Full Text] [supporting information]

42. Chokhawala, H. A.; Cao, H.; Yu, H.; *Chen, X. Enzymatic synthesis of fluorinated mechanistic probes for sialidases and sialyltransferases. J. Am. Chem. Soc. 2007, 129, 10630-10631. [Full Text] [supporting information]

41. Lewis, A. L.; Cao, H.; Patel, S. K.; Diaz, S.; Ryan, W.; Carlin, A. F.; Thon, V.; Lewis, W. G.; Varki, A.; Chen, X.; Nizet, V. NeuA sialic acid O-acetylesterase activity modulates O-acetylation of capsular polysaccharides in Group B Streptococcus. J. Biol. Chem. 2007, 282, 27562-27571. [Full Text]

40. Li, Y.; Sun, M.; Huang, S.; Yu, H.; Chokhawala, H. A.; Thon, V.; *Chen, X. The Hd0053 gene of Haemophilus ducreyi encodes an a2,3-sialyltransferase. Biochem. Biophys. Res. Commun. 2007, 361, 555-560. [Full Text]

39. Yu, H.; Chokhawala, H. A.; Varki, A.; *Chen, X. Efficient chemoenzymatic synthesis of biotinylated human serum albumin-sialoglycoside conjugates containing O-acetylated sialic acids. Org. Biomol. Chem. 2007, 5, 2458-2463. [Full Text]

38. Ni, L.; Chokhawala, H. A.; Cao, H.; Henning, R.; Ng, L.; Huang, S.; Yu, H.; *Chen, X.; *Fisher, A. J. Crystal structures of a Pasteurella multocida sialyltransferase complexes with acceptor and donor analogs reveal substrate binding sites and catalytic mechanism. Biochemistry 2007, 46, 6288-6298. [Full Text]

37. Yu, H.; *Chen, X. Carbohydrate post-glycosylational modifications. Org. Biomol. Chem. 2007, 5, 865-872. [Full Text] [Cover Picture]

36. Huang, S.; Yu, H.; *Chen, X. Disaccharides as unusual sialic acid aldolase substrates: novel synthesis of disaccharides containing a sialic acid at the reducing end. Angew. Chem. Int. Ed. 2007, 46, 2249-2253. [Full Text] [supporting information]

35. Chokhawala, H.; Yu, H.; *Chen X. High-throughput substrate specificity studies of sialidases using chemoenzymatically synthesized sialoside libraries. ChemBioChem 2007, 8, 194-201. [Full Text] [supporting information]

34. Chokhawala, H.; *Chen X. Chemical aspects (scope and limitations) A: Enzymatic approaches to O-glycoside introduction: Glycosyltransferases. In Comprehensive Glycoscience, from Chemistry to Systems Biology (J. P. Kamerling, Ed.) Subject Editors: Geert-Jan Boons, YC Lee, Akemi Suzuki, Naoyuki Taniguchi, and Alphons G. J. Voragen. Elsevier, Oxford, UK, 2007. Invited review. [link]

2006

33. Yu, H.; Chokhawala, H.; Huang, S.; *Chen X. One-pot three-enzyme chemoenzymatic approach to the synthesis of sialosides containing natural and non-natural functionalities. Nature Protocols 2006, 1, 2485-2492. [Abstract]

32. Yu, H.; Huang, S.; Chokhawala, H.; Sun, M.; Zheng, H.; *Chen, X. Highly efficient chemoenzymatic synthesis of naturally occurring and non-natural a2,6-linked sialosides: A P. damsela a2,6-sialyltransferase with extremely flexible donor substrate specificity. Angew. Chem. Int. Ed. 2006, 45, 3938-3944 . [Full Text] [supporting information]

31. Yu, H.; *Chen, X. Aldolase catalyzed synthesis of b-D-Gal p -(1-9)-D-KDN: A novel acceptor for sialyltransferases. Org. Lett. 2006 , 8, 2393-2396. [Full Text] [supporting information]

30. Ni, L., Sun, M., Yu, H., Chokhawala, H., *Chen, X, and *Fisher, A. J. (2006). CMP-Induced Strctural Changes in a Multifunctional Sialyltransferase from Pasteurella multocida . Biochemistry, 2006, 45, 2139-2148. [Full Text]

29. Yu H.;Ryan, W.; Yu, H.; * Chen, X. Characterization of a bifunctional cytidine 5'-monophosphate N -acetylneuramic acid synthestase cloned from Streptococcus agalactiae. Biotech. Lett, 2006, 28, 107-113. [PDF]

2004-2005

28. Yu, H.; Chokhawala, H.; Karpel, R.; Yu, H.; Wu, B.; Zhang, J.; Zhang, Y.; Jia, Q.; *Chen, X. A multifunctional Pasteurella multocida sialyltransferase: a powerful tool for the synthesis of sialoside libraries. J. Am. Chem. Soc, 2005, 127, 17618-17619. [Full Text] [supporting information]

27. Yu, H.; Yu, H.; Karpel, R.; *Chen, X. Chemoenzymatic synthesis of CMP-sialic acid derivatives by a one-pot two-enzyme system: comparison of substrate flexibility of three microbial CMP-sialic acid synthetases. Bioorg. & Med. Chem. , 2004 , 12 , 6427-6435. [PDF]