Publications from Chen's Group

2009    2008    2007    2006    2004-2005

 

2009                                                                                            Back to top

65. Yu, H.; Cheng, J.; Ding, L.; Khedri, Z.; Chen, Y.; Chin, S.; Lau, K.; Tiwari, VK, *Chen, X. Chemoenzymatic synthesis of GD3 oligosaccharides and other disialyl glycans containing natural and non-natural sialic acids. J. Am. Chem. Soc. 2009. Accepted.

64. Cheng, J.; Huang, S.; Yu, H.; Li, Y.; Lau, K.; *Chen, X. Trans-sialidase activity of Photobacterium damsela a2,6-sialyltransferase and its application in the synthesis of sialosides. Glycobiology 2009. In press. doi:10.1093/glycob/CWP172 [Full Text]

63. Pham, T.; Gregg, C.; Karp, F.; Chow, R.; Padler-Karavani, V.; Cao, H.; Chen, X.; Witztum, J. L.; Varki, N. M.; *Varki, A. Evidence for a novel human-specific xeno-auto-antibody response against vascular endothelium. Blood, 2009. In press. PMID: 19828701 [Full Text]

62. Cao, H.; Li, Y.; Lau, K. ; Muthana, S. ; Yu, H.; Cheng, J.; Chokhawala, H. A.; Sugiarto, G.; Zhang, L.; *Chen, X. Sialidase substrate specificity studies using chemoenzymatically synthesized sialosides containing C5-modified sialic acids. Org. Biomol. Chem., 2009. In press. DOI: 10.1039/B916305K [Full Text]

61. Muthana, S.; Cao, H.; *Chen, X. Recent progress in chemical and chemoenzymatic synthesis of carbohydrates. Invited review. Curr. Opin. Chem. Biol., 2009. In press. PMID: 19833544 [Full Text]

60. Cao, H.; Muthana, S.; Li, Y.; Cheng, J.; *Chen, X. Parallel chemoenzymatic synthesis of sialosides containing a C5-diversified sialic acid. Bioorg. Med. Chem. Lett. 2009, 19, 5869-5871. PMID: 19740656. PMC2753693. [Full Text] [supporting information]

59. Linman, M. J.; Yu, H.; Chen, X.; *Cheng, Q. Fabrication and characterization of a sialoside-based carbohydrate microarray biointerface for protein binding analysis with surface Plasmon resonance imaging. ACS Appl. Mater. Interfaces 2009, 1,1755-1762 [Full Text]

58. Muthana, S.; Yu, H.; Cao, H.; Cheng, J.; *Chen, X. Chemoenzymatic synthesis of a new class of macrocyclic oligosaccharides. J. Org. Chem. 2009, 74, 2928-2936. PMID: 19296596. Featured article. [Full text] [supporting information]

57. Yi, W.; Liu, X.; Li, Y.; Li, J.; Xia, C.; Zhou, G.; Zhang, W.; Chen, X.; *Wang, P.G. Remodeling bacterial polysaccharides by metabolic pathway engineering. Proc. Natl. Acad. Sci. U.S.A. 2009, 106, 4207-4212. PMID: 19251666. PMCID: PMC2657399. [Full Text] [supporting information] [Appendix] [News]

56. Diaz, S. L.; Padler-Karavani, V.; Ghaderi, D.; Hurtado-Ziola, N.; Yu, H.; Chen, X.; Brinkman-Van der Linden, E. C. M.; *Varki, A.; Varki, N. M. Sensitive and specific detection of the non-human sialic acid /N/-glycolylneuraminic acid in human tissues and biotherapeutic products. PLoS ONE 2009, 4, e4241 (1-10). Open Access. [Full Text]

55. Wong, J. H.; Sahni, U.; Li, Y.; Chen, X.; *Gervay-Hague, J. Synthesis of sulfone-based nucleotide isosteres: identification of CMP-sialic acid synthetase inhibitors. Org. Biomol. Chem. 2009, 7, 27-29. [Full Text] [supporting information]

54. Yu, H.; *Chen X. Enzymatic synthesis of carbohydrate-containing biomolecules. In Wiley Encyclopedia of Chemical Biology. Chief Advisor: Tadhag P. Begley, John Wiley & Sons, Inc. NJ, January 2009. ISBN: 978-0-471-75477-0. [Link]

2008                                                                                            Back to top

53. *Byres, E.; *Paton, A. W.; Paton, J. C.; Smith, D. F.; Lofling, J. C.; Wilce, M. C. J.; Chong, D. C.; Talbot, U. M.; Yu, H.; Huang, S.; Chen, X.; Varki, N. M.; Varki, A.; Rossjohn, J.; Beddoe, T. Incorporation of a non-human glycan mediates human susceptibility to a bacterial toxin. Nature 2008, 456, 648-652 .[Full Text] [News 1, 2, 3, 4, 5, 6] [supporting information]

52. Chokhawala, H. A.; Huang, S.; Lau, K.; Yu, H.; Cheng, J.; Thon, V.; Hurtado-Ziola, N.; Guerrero, J.; Varki, A.; *Chen, X. Combinatorial chemoenzymatic synthesis and high-throughput screening of sialosides. ACS Chemical Biology 2008, 3(9), 567-576. [Full Text] [supporting information]

51. Padler-Karavani, V.; Yu, H.; Cao, H.; Chokhawala, H. A.; Karp, F.; Chen, X.; Varki, N.; *Varki, A. Diversity in specificity, abundance and composition of anti-Neu5Gc antibodies in normal humans: Potential implications for disease. Glycobiology 2008, 18, 818-830. [Full Text]

50. Cao, H.; Huang, S.; Cheng, J.; Li, Y.; Muthana, S.; Son, B.; *Chen, X. Chemical preparation of sialyl Lewis x using an enzymatically synthesized sialoside building block. Carbohydr. Res.2008, 343, 2863-2869. PMID: 18639240. [Full Text]

49. Cheng, J.; Yu, H.; Lau, K.; Huang, S.; Chokhawala, H. A.; Li, Y.; Tiwari, V. K.; *Chen, X. Multifunctionality of Campylobacter jejuni sialyltransferase CstII: Characterization of GD3/GT3 oligosaccharide synthase, GD3 oligosaccharide sialidase and trans-sialidase activities. Glycobiology 2008, 18, 686-697. [Full Text]

48. Wang, Z.; Gilbert, M.; Eguchi, H.; Yu, H.; Cheng, J.; Muthana, S.; Zhou, L.; Wang, P. G.; Chen, X.; *Huang, X. Chemoenzymatic synthesis of tumor-associated carbohydrate antigen Globo-H and stage-specific embryonic antigen 4. Adv. Synth. Catal. 2008, 350, 1717-1728. [Full Text]

47. Yu, H.; Chokhawala, H. A.; Huang, S.; *Chen, X. Chemoenzymatic synthesis of sialosides and their applications. In Chemical Glycobiology ACS book series, Chen, X.; Halcomb, R.; Wang, P. G. Eds 990, Chen, X.; Halcomb, R.; Wang, P. G. Eds. 2008, Oxford University Press, Page 96-122. [Link]

46. Li, Y.; Yu, H.; Cao, H.; Lau, K.; Muthana, S.; Tiwari, V. K.; Son, B., *Chen, X. Pasteurella multocida sialic acid aldolase: a promising biocatalyst. Appl. Microbiol. Biotech. 2008, 79, 963-970. [Full Text]

45. Linman, M. J.; Taylor, J. D.; Yu, H.; Chen, X.; *Cheng, Q. Surface Plasmon Resonance study of protein-carbohydrate interactions using biotinylated sialosides. Anal. Chem. 2008, 80, 4007-4013. [Full Text]

44. Sun, M.; Li, Y.; Chokhawala, H. A.; Henning, R.; *Chen, X. N-terminal 112 amino acid residues are not required for the sialyltransferase activity of /Photobacterium damsela/ a2,6-sialyltransferase. Biotechnol. Lett. 2008, 30, 671-676.[Full Text]

2007                                                                                           Back to top

43. Muthana, S.; Yu, H.; Huang, S.; *Chen, X. Chemoenzymatic synthesis of size-defined polysaccharides by sialyltransferase-catalyzed block transfer of oligosaccharides. J. Am. Chem. Soc. 2007, 129, 11918-11919. [Full Text] [supporting information]

42. Chokhawala, H. A.; Cao, H.; Yu, H.; *Chen, X. Enzymatic synthesis of fluorinated mechanistic probes for sialidases and sialyltransferases. J. Am. Chem. Soc. 2007, 129, 10630-10631. [Full Text] [supporting information]

41. Lewis, A. L.; Cao, H.; Patel, S. K.; Diaz, S.; Ryan, W.; Carlin, A. F.; Thon, V.; Lewis, W. G.; Varki, A.; Chen, X.; Nizet, V. NeuA sialic acid O-acetylesterase activity modulates O-acetylation of capsular polysaccharides in Group B Streptococcus. J. Biol. Chem. 2007, 282, 27562-27571. [Full Text]

40. Li, Y.; Sun, M.; Huang, S.; Yu, H.; Chokhawala, H. A.; Thon, V.; *Chen, X. The Hd0053 gene of Haemophilus ducreyi encodes an a2,3-sialyltransferase. Biochem. Biophys. Res. Commun. 2007, 361, 555-560. [Full Text]

39. Yu, H.; Chokhawala, H. A.; Varki, A.; *Chen, X. Efficient chemoenzymatic synthesis of biotinylated human serum albumin-sialoglycoside conjugates containing O-acetylated sialic acids. Org. Biomol. Chem. 2007, 5, 2458-2463. [Full Text]

38. Ni, L.; Chokhawala, H. A.; Cao, H.; Henning, R.; Ng, L.; Huang, S.; Yu, H.; *Chen, X.; *Fisher, A. J. Crystal structures of a Pasteurella multocida sialyltransferase complexes with acceptor and donor analogs reveal substrate binding sites and catalytic mechanism. Biochemistry 2007, 46, 6288-6298. [Full Text]

37. Yu, H.; *Chen, X. Carbohydrate post-glycosylational modifications. Org. Biomol. Chem. 2007, 5, 865-872. [Full Text] [Cover Picture]

36. Huang, S.; Yu, H.; *Chen, X. Disaccharides as unusual sialic acid aldolase substrates: novel synthesis of disaccharides containing a sialic acid at the reducing end. Angew. Chem. Int. Ed. 2007, 46, 2249-2253. [Full Text] [supporting information]

35. Chokhawala, H.; Yu, H.; *Chen X. High-throughput substrate specificity studies of sialidases using chemoenzymatically synthesized sialoside libraries. ChemBioChem 2007, 8, 194-201. [Full Text] [supporting information]

34. Chokhawala, H.; *Chen X. Chemical aspects (scope and limitations) A: Enzymatic approaches to O-glycoside introduction: Glycosyltransferases. In Comprehensive Glycoscience, from Chemistry to Systems Biology (J. P. Kamerling, Ed.) Subject Editors: Geert-Jan Boons, YC Lee, Akemi Suzuki, Naoyuki Taniguchi, and Alphons G. J. Voragen. Elsevier, Oxford, UK, 2007. Invited review. [link]

2006                                                                                           Back to top

33. Yu, H.; Chokhawala, H.; Huang, S.; *Chen X. One-pot three-enzyme chemoenzymatic approach to the synthesis of sialosides containing natural and non-natural functionalities. Nature Protocols 2006, 1, 2485-2492. [Abstract]

32. Yu, H.; Huang, S.; Chokhawala, H.; Sun, M.; Zheng, H.; *Chen, X. Highly efficient chemoenzymatic synthesis of naturally occurring and non-natural a2,6-linked sialosides: A P. damsela a2,6-sialyltransferase with extremely flexible donor substrate specificity. Angew. Chem. Int. Ed. 2006, 45, 3938-3944 . [Full Text] [supporting information]

31. Yu, H.; *Chen, X. Aldolase catalyzed synthesis of b-D-Gal p -(1-9)-D-KDN: A novel acceptor for sialyltransferases. Org. Lett. 2006 , 8, 2393-2396. [Full Text] [supporting information]

30. Ni, L., Sun, M., Yu, H., Chokhawala, H., *Chen, X, and *Fisher, A. J. (2006). CMP-Induced Strctural Changes in a Multifunctional Sialyltransferase from Pasteurella multocida . Biochemistry, 2006, 45, 2139-2148. [Full Text]

29. Yu H.;Ryan, W.; Yu, H.; * Chen, X. Characterization of a bifunctional cytidine 5'-monophosphate N -acetylneuramic acid synthestase cloned from Streptococcus agalactiae. Biotech. Lett, 2006, 28, 107-113. [Full Text]

2004-2005                                                                                   Back to top

28. Yu, H.; Chokhawala, H.; Karpel, R.; Yu, H.; Wu, B.; Zhang, J.; Zhang, Y.; Jia, Q.; *Chen, X. A multifunctional Pasteurella multocida sialyltransferase: a powerful tool for the synthesis of sialoside libraries. J. Am. Chem. Soc, 2005, 127, 17618-17619. [Full Text] [supporting information]

27. Yu, H.; Yu, H.; Karpel, R.; *Chen, X. Chemoenzymatic synthesis of CMP-sialic acid derivatives by a one-pot two-enzyme system: comparison of substrate flexibility of three microbial CMP-sialic acid synthetases. Bioorg. & Med. Chem. , 2004 , 12 , 6427-6435. [Full Text]