Publications |
|
2012 2011 2010 2009 2008 2007 2006 2004-2005 Previous Patent Applications Book Edited 99. ^Khedri, Z.; ^Muthana, M. M.; ^Li, Y.; Muthana, S.; Yu, H.; Cao, H.; *Chen, X. Probe sialidase substrate specificity using chemoenzymatically synthesized sialosides containing C9-modified sialic acids. Chem. Commun. 2012, In press. DOI:10.1039/C2CC17393J ^Co-first authors. 98. ^Muthana, M. M.; ^Qu, J.; Li, Y.; Zhang, L.; Yu, H.; Ding, L.; Malekan, H.; *Chen, X. Efficient one-pot multienzyme synthesis of UDP-sugars using a promiscuous UDP-sugar pyrophosphorylase from Bifidobacterium longum (BLUSP). Chem. Commun. 2012, 48, 2728-2730. PMID: 22306833 ^Co-first authors. 97. Thon, V.; Li, Y.; Yu, H.; Lau, K.; *Chen, X. PmST3 from Pasteurella multocida encoded by Pm1174 gene is a monofunctional alpha 2-3- sialyltransferase. Appl. Microbiol. Biotechnol. 2012, In press. PMID: 22075637. 96. Li, Y.; Yu, H.; Cao, H.; Muthana, S.; *Chen, X. Pasteurella multocida CMP-sialic acid synthetase and mutants of Neisseria meningitidis CMP-sialic acid synthetase with improved substrate promiscuity. Appl. Microbiol. Biotechnol. 2012, In press. PMID: 21968653. [Full Text] [Supporting information] 95. Linman, M. J.; Yu, H.; Chen, X.; Cheng, Q. Chapter 13. Surface plasmon resonance imaging analysis of protein binding to a sialoside-focused carbohydrate microarray. In Methods in Molecular Biology series Carbohydrate Microarrays: Methods and Protocols. Edited by Yann Chevolot. 2012, 808, 183-194. PMID: 22057526. 94. *Cao, H.; *Chen, X. Chapter 3. General consideration on sialic acid chemistry. In Methods in Molecular Biology series Carbohydrate Microarrays: Methods and Protocols. Edited by Yann Chevolot. 2012, 808, 31-56. PMID: 22057516. 2011 Back to top 93. Fei, Y.; Sun, Y.-S.; Li, Y.; Lau, K.; Yu, H.; Chokhawala, H. A.; Huang, S.; Landry, J. P.; Chen, X.; *Zhu, X. Fluorescent labelling agents change affinity profiles of glycan-binding proteins. Mol. BioSyst. 2011, 7, 3343-3352. PMID: 22009201. PMCID: PMC327173292. 92. Bradley, K. C.; Galloway, S. E.; Lasanajak, Y.; Song, X.; Heimburg-Molinaro, J.; Talekar, G. R.; Yu, H.; Chen, X.; Smith, D. F.; Cummings, R. D.; *Steinhauer, D. A. Analysis of influenza virus hemagglutinin receptor binding mutants with limited receptor recognition properties and conditional replication characteristics. J. Virol. 2011, 85, 12387-12398. PMID: 21917953. PMCID: PMC3209400 91. Chen, Y.; Thon, V.; Li, Y.; Yu, H.; Ding, L.; Lau, K.; Qu, J.; Hie, L.; *Chen, X. One-pot three-enzyme synthesis of UDP-GlcNAc derivatives. Chem. Comm. (Camb) 2011, 47, 10815-10817. PMID: 21863157. DOI:10.1039/C1CC14034E.[Full Text] [Supporting information] 90. Sugiarto, G., Lau, K.; Li, Y.; Khedri, Z.; Yu, H.; Le, D.-T.; *Chen, X. Decreasing the sialidase activity of multifunctional Pasteurella multocida alpha 2-3-sialyltransferase 1 (PmST1) by site-directed mutagenesis. Mol. BioSyst. 2011, , 7, 3021-3027. PMID: 21858283. DOI:10.1039/C1MB05182B. [Full Text] 89. Li, Y.; Yu, H.; Chen, Y.; Lau, K.; Cai, L.; Cao, H.; Tiwari, V. K.; Qu, J.; Thon, V.; Wang, P. G.; *Chen, X. Substrate promiscuity of N-acetylhexosamine 1-kinases. Molecules 2011, 16, 6396-6407. Special Issue "Enzyme-catalyzed reactions". PMID: 21799473. [Full Text] 88. Song, X.; Yu, H.; Chen, X.; Lasanajak, Y.; Tappert, M. M.; Air, G. M.; Tiwari, V. K.; Cao, H.; Chokhawala, H. A.; Zheng, H.; *Cummings, R. D.; *Smith, D. F. A sialylated glycan microarray reveals novel interactions of modified sialic acids with proteins and viruses. J. Biol. Chem. 2011, In press. PMID: 21757734. PMCID: PMC3173124 [Full Text] 87. Ding, L.; Yu, H.; Lau, K.; Li, Y.; Muthana, S.; Wang, J.; *Chen, X. Efficient chemoenzymatic synthesis of sialyl Tn antigen and derivatives. Chem. Comm. 2011, 47, 8691-8693. PMID: 21725542. Web-themed issue on Glycochemistry & Glycobiology. PMCID: PMC3201767. [Full Text] [Supporting Information] 86. Urban, I.; Santurio, L. B.; Chidichimo, A.; Yu, H.; Chen, X.; Mucci, J.; Aguero, F.; *Buscaglia, C. A. Molecular diversity of the Trypanosoma cruzi TcSMUG family of mucin genes and proteins. Biochem. J. 2011, 438, 303-313. PMID: 21651499. [Full Text] 85. Pourceau, G.; *Chevolot, Y.; Goudot, A.; Giroux, F.; Meyer, A.; Moules, V.; Lina, B.; Cecioni, S.; Vidal, S.; Yu, H.; Chen, X.; Ferraris, O.; Praly, J.-P.; Souteyrand, E.; Vasseur, J.-J.; Morvan, F. Measurement of enzymatic activity and specificity of human and avian influenza neuraminidases from whole virus by glycoarray and MALDI-TOF mass spectrometry. ChemBioChem 2011, 12, 2071-2080. PMID: 21739555. [Full Text] [Supporting Information] 84. Thon, V.; Lau, K.; Yu, H.; Tran, B. K.; *Chen, X. PmST2 - A novel Pasteurella multocida glycolipids alpha 2-3-sialyltransferase. Glycobiology 2011, 21, 1206-1216. PMID: 21515586. PMCID: PMC3150114. [Full Text] [Supporting Information] 83. Yu, H.; Cao, H.; Tiwari, V. K.; Li, Y.; *Chen, X. Chemoenzymatic synthesis of C8-modified sialic acids and related alpha 2-3- and alpha 2-6-linked sialosides. Bioorg. Med. Chem. Lett. 2011, 21, 5037-5040. PMID: 21592790. PMCID: PMC3156331. doi: 10.1016/j.bmcl.2011.04.083. [Full Text] 82. Li, Y.; Cao, H.; Dao, N.; Luo, Z.; Yu, H.; Chen, Y.; Baumgarth, N.; Cardona, C.; *Chen, X. High-throughput neuraminidase substrate specificity study of human and avian influenza viruses. Virology 2011, 415, 12-19. PMID: 21501853. PMCID: PMC3114948. [Full Text] 81. Chen, G.-Y.; Chen, X.; King, S.; Cavassani, K. A.; Cheng, J.; Zheng, X.; Cao, H.; Yu, H.; Qu, J.; Fang, D.; Wu, W.; Bai, X.-F.; Liu, J.-Q.; Woodiga, S. A.; Chen, C.; Sun, L.; Hogaboam, C. M.; Kunkel, S. L.; *Zheng, P.; *Liu, Y. Amelioration of sepsis by inhibiting sialidase-mediated disruption of CD24-Siglec G interaction. Nat. Biotechnol. 2011, 29, 428-435. PMID: 21478876. [Full Text] [Supporting information] 80. Padler-Karavani, V.; Hurtado-Ziola, N.; Pu, M.; Yu, H.; Huang, S.; Muthana, S.; Chokhawala, H. A.; Cao, H.; Secrest, P.; Friedmann-Morvinski, D.; Singer, O.; Ghaderi, D.; Verma, I. M.; Liu, Y.-T.; Messer, K.; Chen, X., *Varki, A., Schwab, R. Human xeno-autoantibodies against a non-human sialic acid serve as novel serum biomarkers and immunotherapeutics in cancer. Cancer Res. 2011, 71, 3352-3363. PMID: 21505105.PMCID: PMC3085609. [Full Text] [Supporting information] 79. Sela, D. A.; Li, Y.; Lerno, L.; Wu, S.; Marcobal, A. M.; German, B. J.; Chen, X.; Lebrilla, C. B.; *Mills, D. A. An infant-associated bacterial commensal utilizes human milk sialyloligosaccharides. J. Biol. Chem. 2011, 286, 11909-11918. PMID: 21288901.PMCID: PMC3069393. [Full Text] [supporting information] 78. Lau, K.; Yu, H.; Thon, V.; Khedri, Z.; Leon, M. E.; Tran, B. K.; *Chen, X. Sequential two-step multienzyme synthesis of sialyl T antigens and derivatives. Org. Biomol. Chem. 2011, 9, 2784-2789. PMID: 21359399. PMCID: PMC3114947. [Full Text] [supporting information] 77. *Cao, H.; Hwang, J.; *Chen, X. Carbohydrate-containing natural products in medicinal chemistry. In Opportunity, Challenge and Scope of Natural Products in Medicinal Chemistry. Vinod K. Tiwari Ed.2011, 411-431. [Full Text] 76. Yu, H.; *Chen, X. Combinatorial biosynthesis of complex carbohydrates. Chapter 10 in Carbohydrate Recognition: Biological Problems, Methods, and Applications. Binghe Wang, Geert-Jan Boons Eds. 2011, 229-255. Wiley. [Link] 75. Huang, S.; Yu, H.; *Chen, X. Efficient chemoenzymatic synthesis of sialosides. Science China Chemistry. 2011, 54, 117-128. PMID: 21686057. PMCID: PMC3115702. [Full Text] 74. *Chen, X. Fermenting next generation glycosylated therapeutics. ACS Chem. Biol. An invited Point of View report. 2011, 6, 14-17. PMID: 21250649. [Full Text] 73. Sugiarto, G.; Lau, K.; Yu, H.; Vuong, S.; Thon, V.; Li, Y.; Huang S.; *Chen, X. Cloning and characterization of a viral alpha2-3-sialyltransferase (vST3Gal-I) for the synthesis of sialyl Lewis x. Glycobiology 2011, 21, 387-396. PMID: 20978012. PMCID: PMC3033747 [Full Text] [supporting information] 72. Li, Y.; Cao, H.; Yu, H.; Chen, Y.; Lau, K.; Qu, J.; Thon, V.; Sugiarto, G. *Chen, X. Identifying specific sialidase inhibitors for human cytosolic sialidase NEU2 by substrate specificity studies. Mol. Biosyst. 2011,7, 1060-1072. PMID: 21206954.PMCID: PMC3114945. [Full Text] [supporting information]
2010 Back to top 71. Yu, H.; Thon, V.; Lau, K.; Cai, L.; Chen, Y.; Mu, S.; Li, Y.; Wang, P. G.; *Chen, X. Highly efficient chemoenzymatic synthesis of beta1-3-linked galactosides. Chem. Comm. 2010, 46, 7507-7509. PMID: 20830443. DOI: 10.1039/C0CC02850A [Full Text] [supporting information] 70. Taylor, R. E.; Gregg, C.; Padler-Karavani, V.; Ghaderi, D.; Yu, H.; Huang, S.; Sorensen, R. U.; Chen, X.; Inostroza, J.; Nizet, V.; Varki, A. Novel mechanism for the generation of human xeno-auto-antibodies against the non-human sialic acid N-glycolylneuraminic acid. J. Exp. Med.-Brief Definitive Report. 2010, 207, 1637-1646. PMID: 20624889.[Full Text] 69. Lau, K.; Thon, V.; Yu, H.; Ding, L.; Chen, Y.; Muthana, M. M.; Wong, D.; Huang, R.; *Chen, X. Highly efficient chemoenzymatic synthesis of beta1-4-linked galactosides with promiscuous bacterial beta1-4-galactosyltransferases. Chem Comm. 2010, 46, 6066-6068. PMID: 20625591. DOI:10.1039/C0CC01381A. [Full Text] [supporting information] 68. Zhang, L.; Lau, K.; Cheng, J.; Yu, H.; Li, Y.; Sugiarto, G.; Huang, S.; Ding, L.; Thon, V.; Wang, P. G.; *Chen, X. Helicobacter hepaticus Hh0072 gene encodes a novel alpha1-3-fucosyltransferase belonging to CAZy GT11 family. Glycobiology 2010, 20, 1077-1088. PMID: 20466652. [Full Text] 67. Muia, R. P.; Yu, H.; Prescher, J. A.; Hellman, U.; Chen, X.; Bertozzi, C. R.; *Campetella, O. Identification of glycoproteins targeted by Trypanosoma cruzi trans-sialidase, a virulence factor that disturbs lymphocyte glycosylation. Glycobiology 2010, 20, 833-842. PMID: 20354005 [Full Text] 66. *Chen, X.; *Varki, A. Advances in sialic acid biology and chemistry. ACS Chem. Biol. 2010, 5, 163-176. PMID: 20020717. PMCID: PMC2825284 DOI: 10.1021/cb900266r [Full Text] 65. Cheng, J.; Huang, S.; Yu, H.; Li, Y.; Lau, K.; *Chen, X. Trans-sialidase activity of Photobacterium damsela alpha2,6-sialyltransferase and its application in the synthesis of sialosides. Glycobiology 2010, 20, 260-268. PMID: 19880425.. DOI:10.1093/glycob/CWP172 [Full Text] 2009 Back to top 64. Yu, H.; Cheng, J.; Ding, L.; Khedri, Z.; Chen, Y.; Chin, S.; Lau, K.; Tiwari, VK, *Chen, X. Chemoenzymatic synthesis of GD3 oligosaccharides and other disialyl glycans containing natural and non-natural sialic acids. J. Am. Chem. Soc. 2009, 131, 18467-18477. PMID: 19947630. DOI: 10.1021/ja907750r [Full Text] 63. Pham, T.; Gregg, C.; Karp, F.; Chow, R.; Padler-Karavani, V.; Cao, H.; Chen, X.; Witztum, J. L.; Varki, N. M.; *Varki, A. Evidence for a novel human-specific xeno-auto-antibody response against vascular endothelium. Blood, 2009, 114, 5225-5235. PMID: 19828701. DOI 10.1182/blood-2009-05-220400 [Full Text] 59. Linman, M. J.; Yu, H.; Chen, X.; *Cheng, Q. Fabrication and characterization of a sialoside-based carbohydrate microarray biointerface for protein binding analysis with surface Plasmon resonance imaging. ACS Appl. Mater. Interfaces 2009, 1,1755-1762 [Full Text] 58. Muthana, S.; Yu, H.; Cao, H.; Cheng, J.; *Chen, X. Chemoenzymatic synthesis of a new class of macrocyclic oligosaccharides. J. Org. Chem. 2009, 74, 2928-2936. PMID: 19296596. Featured article. [Full text] [supporting information] 55. Wong, J. H.; Sahni, U.; Li, Y.; Chen, X.; *Gervay-Hague, J. Synthesis of sulfone-based nucleotide isosteres: identification of CMP-sialic acid synthetase inhibitors. Org. Biomol. Chem. 2009, 7, 27-29. [Full Text] [supporting information] 54. Yu, H.; *Chen X. Enzymatic synthesis of carbohydrate-containing biomolecules. In Wiley Encyclopedia of Chemical Biology. Chief Advisor: Tadhag P. Begley, John Wiley & Sons, Inc. NJ, 2009, 1, 631-652. ISBN: 978-0-471-75477-0. [Link] 2008 Back to top 53. *Byres, E.; *Paton, A. W.; Paton, J. C.; Smith, D. F.; Lofling, J. C.; Wilce, M. C. J.; Chong, D. C.; Talbot, U. M.; Yu, H.; Huang, S.; Chen, X.; Varki, N. M.; Varki, A.; Rossjohn, J.; Beddoe, T. Incorporation of a non-human glycan mediates human susceptibility to a bacterial toxin. Nature 2008, 456, 648-652 .[Full Text] [News 1, 2, 3, 4, 5, 6] [supporting information] 52. Chokhawala, H. A.; Huang, S.; Lau, K.; Yu, H.; Cheng, J.; Thon, V.; Hurtado-Ziola, N.; Guerrero, J.; Varki, A.; *Chen, X. Combinatorial chemoenzymatic synthesis and high-throughput screening of sialosides. ACS Chemical Biology 2008, 3(9), 567-576. [Full Text] [supporting information] 51. Padler-Karavani, V.; Yu, H.; Cao, H.; Chokhawala, H. A.; Karp, F.; Chen, X.; Varki, N.; *Varki, A. Diversity in specificity, abundance and composition of anti-Neu5Gc antibodies in normal humans: Potential implications for disease. Glycobiology 2008, 18, 818-830. [Full Text] 50. Cao, H.; Huang, S.; Cheng, J.; Li, Y.; Muthana, S.; Son, B.; *Chen, X. Chemical preparation of sialyl Lewis x using an enzymatically synthesized sialoside building block. Carbohydr. Res.2008, 343, 2863-2869. PMID: 18639240. [Full Text] 49. Cheng, J.; Yu, H.; Lau, K.; Huang, S.; Chokhawala, H. A.; Li, Y.; Tiwari, V. K.; *Chen, X. Multifunctionality of Campylobacter jejuni sialyltransferase CstII: Characterization of GD3/GT3 oligosaccharide synthase, GD3 oligosaccharide sialidase and trans-sialidase activities. Glycobiology 2008, 18, 686-697. [Full Text] 48. Wang, Z.; Gilbert, M.; Eguchi, H.; Yu, H.; Cheng, J.; Muthana, S.; Zhou, L.; Wang, P. G.; Chen, X.; *Huang, X. Chemoenzymatic synthesis of tumor-associated carbohydrate antigen Globo-H and stage-specific embryonic antigen 4. Adv. Synth. Catal. 2008, 350, 1717-1728. [Full Text] 47. Yu, H.; Chokhawala, H. A.; Huang, S.; *Chen, X. Chemoenzymatic synthesis of sialosides and their applications. In Chemical Glycobiology ACS book series, Chen, X.; Halcomb, R.; Wang, P. G. Eds 990, Chen, X.; Halcomb, R.; Wang, P. G. Eds. 2008, Oxford University Press, Page 96-122. [Link] 46. Li, Y.; Yu, H.; Cao, H.; Lau, K.; Muthana, S.; Tiwari, V. K.; Son, B., *Chen, X. Pasteurella multocida sialic acid aldolase: a promising biocatalyst. Appl. Microbiol. Biotech. 2008, 79, 963-970. [Full Text] 45. Linman, M. J.; Taylor, J. D.; Yu, H.; Chen, X.; *Cheng, Q. Surface Plasmon Resonance study of protein-carbohydrate interactions using biotinylated sialosides. Anal. Chem. 2008, 80, 4007-4013. [Full Text] 44. Sun, M.; Li, Y.; Chokhawala, H. A.; Henning, R.; *Chen, X. N-terminal 112 amino acid residues are not required for the sialyltransferase activity of Photobacterium damsela alpha2,6-sialyltransferase. Biotechnol. Lett. 2008, 30, 671-676.[Full Text] 2007 Back to top 43. Muthana, S.; Yu, H.; Huang, S.; *Chen, X. Chemoenzymatic synthesis of size-defined polysaccharides by sialyltransferase-catalyzed block transfer of oligosaccharides. J. Am. Chem. Soc. 2007, 129, 11918-11919. [Full Text] [supporting information] 42. Chokhawala, H. A.; Cao, H.; Yu, H.; *Chen, X. Enzymatic synthesis of fluorinated mechanistic probes for sialidases and sialyltransferases. J. Am. Chem. Soc. 2007, 129, 10630-10631. [Full Text] [supporting information] 41. Lewis, A. L.; Cao, H.; Patel, S. K.; Diaz, S.; Ryan, W.; Carlin, A. F.; Thon, V.; Lewis, W. G.; Varki, A.; Chen, X.; Nizet, V. NeuA sialic acid O-acetylesterase activity modulates O-acetylation of capsular polysaccharides in Group B Streptococcus. J. Biol. Chem. 2007, 282, 27562-27571. [Full Text] 40. Li, Y.; Sun, M.; Huang, S.; Yu, H.; Chokhawala, H. A.; Thon, V.; *Chen, X. The Hd0053 gene of Haemophilus ducreyi encodes an alpha2,3-sialyltransferase. Biochem. Biophys. Res. Commun. 2007, 361, 555-560. [Full Text] 39. Yu, H.; Chokhawala, H. A.; Varki, A.; *Chen, X. Efficient chemoenzymatic synthesis of biotinylated human serum albumin-sialoglycoside conjugates containing O-acetylated sialic acids. Org. Biomol. Chem. 2007, 5, 2458-2463. [Full Text] 38. Ni, L.; Chokhawala, H. A.; Cao, H.; Henning, R.; Ng, L.; Huang, S.; Yu, H.; *Chen, X.; *Fisher, A. J. Crystal structures of a Pasteurella multocida sialyltransferase complexes with acceptor and donor analogs reveal substrate binding sites and catalytic mechanism. Biochemistry 2007, 46, 6288-6298. [Full Text] 37. Yu, H.; *Chen, X. Carbohydrate post-glycosylational modifications. Org. Biomol. Chem. 2007, 5, 865-872. [Full Text] [Cover Picture] 36. Huang, S.; Yu, H.; *Chen, X. Disaccharides as unusual sialic acid aldolase substrates: novel synthesis of disaccharides containing a sialic acid at the reducing end. Angew. Chem. Int. Ed. 2007, 46, 2249-2253. [Full Text] [supporting information] 35. Chokhawala, H.; Yu, H.; *Chen X. High-throughput substrate specificity studies of sialidases using chemoenzymatically synthesized sialoside libraries. ChemBioChem 2007, 8, 194-201. [Full Text] [supporting information] 34. Chokhawala, H.; *Chen X. Chemical aspects (scope and limitations) A: Enzymatic approaches to O-glycoside introduction: Glycosyltransferases. In Comprehensive Glycoscience, from Chemistry to Systems Biology (J. P. Kamerling, Ed.) Subject Editors: Geert-Jan Boons, YC Lee, Akemi Suzuki, Naoyuki Taniguchi, and Alphons G. J. Voragen. Elsevier, Oxford, UK, 2007. Invited review. 2007, 1, 415-451. [link] 2006 Back to top 33. Yu, H.; Chokhawala, H.; Huang, S.; *Chen X. One-pot three-enzyme chemoenzymatic approach to the synthesis of sialosides containing natural and non-natural functionalities. Nature Protocols 2006, 1, 2485-2492. [Abstract] 32. Yu, H.; Huang, S.; Chokhawala, H.; Sun, M.; Zheng, H.; *Chen, X. Highly efficient chemoenzymatic synthesis of naturally occurring and non-natural alpha2,6-linked sialosides: A P. damsela alpha2,6-sialyltransferase with extremely flexible donor substrate specificity. Angew. Chem. Int. Ed. 2006, 45, 3938-3944 . [Full Text] [supporting information] 30. Ni, L., Sun, M., Yu, H., Chokhawala, H., *Chen, X, and *Fisher, A. J. (2006). CMP-Induced Strctural Changes in a Multifunctional Sialyltransferase from Pasteurella multocida . Biochemistry, 2006, 45, 2139-2148. [Full Text] 29. Yu H.;Ryan, W.; Yu, H.; * Chen, X. Characterization of a bifunctional cytidine 5'-monophosphate N -acetylneuramic acid synthestase cloned from Streptococcus agalactiae. Biotech. Lett, 2006, 28, 107-113. [Full Text] 2004-2005 Back to top 28. Yu, H.; Chokhawala, H.; Karpel, R.; Yu, H.; Wu, B.; Zhang, J.; Zhang, Y.; Jia, Q.; *Chen, X. A multifunctional Pasteurella multocida sialyltransferase: a powerful tool for the synthesis of sialoside libraries. J. Am. Chem. Soc, 2005, 127, 17618-17619. [Full Text] [supporting information] 27. Yu, H.; Yu, H.; Karpel, R.; *Chen, X. Chemoenzymatic synthesis of CMP-sialic acid derivatives by a one-pot two-enzyme system: comparison of substrate flexibility of three microbial CMP-sialic acid synthetases. Bioorg. & Med. Chem. , 2004 , 12 , 6427-6435. [Full Text] |